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CELL CULTURE:
It is an in vitro growth of cells although the cells proliferate they do not organize into tissue.
ANAEROBIC CULTURE:
- It is the culture which is carried out in the absence of air.
- Based on the requirement of oxygen, the microbes are broadly classified as Aerobic and Anerobic. Aerobic organisms needs oxygen whereas, anaerobic microorganisms do not need oxygen.
- As oxygen is ubiquitous in air, retaining O2-free conditions during all steps of cultivation is challenging but a prerequisite for anaerobic culturing.
- Most strict anaerobes require not only the absence of oxygen to initiate growth, but also a redox potential below -300mV, which can be only achieved by the supplementation of media with reducing agents.
- Thioglycollate broth is a multi-purpose, enriched differential medium used primarily to determine the oxygen requirements of microorganisms.
PURE CULTURE:
It is a culture of a single cell species, without presence of any contaminants.
PRIMARY CULTURE:
Refers to cultures prepared from tissues taken directly from animals.
SECONDARY CULTURE:
It refers to a subculture derived from a primary culture.
PLATE CULTURE:
It refers to the culture which is grown on a medium, usually agar or gelatin, on a Petri dish.
STREAK CULTURE:
It refers to the culture one in which the medium is inoculated by drawing an infected wire across it.
SUSPENSION CULTURE:
It refers to a culture in which cells multiply while suspended in a suitable medium.
SLANT CULTURE:
This culture is made on the surface of solidified medium in a tube which has been tilted to provide a greater surface area for growth.
STAB CULTURE:
In this culture, the medium is inoculated by thrusting a needle deep into its substance.
TISSUE CULTURE
It refers to the culture in which maintenance or growth of tissue, organ primordia, or the whole or part of an organ in vitro is done so as to preserve its architecture and function.
