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1. NAD is not consumed during which of the following reaction is krebs cycle
- A-ketoglutarate dehydrogenase
- Succinate dehydrogenase
- Malate dehydrogenase
- Isocitrate dehydrogenase
ANSWER b
In eukaryotes, the citric acid cycle takes place in the matrix of the mitochondria, just like the conversion of pyruvate to acetyl CoA
In prokaryotes, these steps both take place in the cytoplasm. The citric acid cycle is a closed loop; the last part of the pathway reforms the molecule used in the first step.
2. Which of the following enzyme are found in CSF
- GGT +ALP
- ALP+CK-MB
- CK+LDH
- Deaminase and peroxidase
ANSWER c
The diagnostic value of cerebrospinal fluid (CSF) enzyme activities in neurological disorders has been evaluated most extensively with the enzymes
- Aspartate aminotransferase (ASAT),
- Lactate dehydrogenase (LDH),
- Creatine kinase (CK) and
- Lysozyme.
3. Western blot technique is used to identify which of the following
- DNA
- RNA
- mRNA
- Proteins
ANSWER d
Western blot is often used in research to separate and identify proteins.
In this technique a mixture of proteins is separated based on molecular weight,
and thus by type, through gel electrophoresis.
These results are then transferred to a membrane producing a band for each protein.
The membrane is then incubated with labels antibodies specific to the protein of interest.
4. Not a component of PCR
- DNA template
- Dideoxynucleotide
- Deoxynucleotide
- DNA polymerase
ANSWER b
5 basic components are required for the PCR process. These are:
- DNA template,
- DNA polymerase enzyme (or Taq polymerase enzyme),
- Primers,
- Nucleotides, and
- Reaction buffers.
5. Cells differentiation by surface markers
- G banding
- Electrophoresis
- Spectrometry
- Flowcytometry
ANSWER b
The fundamental principle of electrophoresis is the existence of charge
separation between the surface of a particle and the fluid immediately
surrounding it. An applied electric field acts on the resulting charge density,
causing the particle to migrate and the fluid around the particle to flow.
Electrophoresis is a laboratory technique used to separate DNA, RNA or protein
molecules based on their size and electrical charge.
