The dentinoenamel junction (DEJ) is a scalloped border between the dentin
and the enamel with the convexity of the scallop directed towards the dentin.
Its scalloped nature increases the surface area of contact between the enamel
and the dentin and reduces the incidence of cracks developing along the DEJ.
The DEJ is less mineralized than the enamel and the dentin.
Enamel Lamellae
Enamel lamellae are hypocalcified leaf-like structures extending from the
outer surface of enamel towards the DEJ.
These are formed due to stress occurring during enamel formation or due to
cracks which develop in the enamel after eruption of teeth.
These contain salivary components and organic debris.
These are considered weak points in enamel and may allow passage of
microorganisms which enter and initiate dental caries.
Enamel Tufts
Enamel tufts are hypocalcified structures appearing like tufts of grass at the
DEJ.
They extend up to one-thirds of the thickness of the enamel from the DEJ.
These are areas in mature enamel where the highest protein content is
present.
Enamel Spindles
Enamel spindles are odontoblastic processes which pass across the DEJ into
the enamel and become embedded in the enamel matrix.
These appear as dark spindle-shaped structures due to thickening of their
ends.
These are more abundant in the cuspal region.
These are easily identifiable in longitudinal section due to their alignment.
Incremental Lines of Retzius
The incremental lines of Retzius indicate the incremental or rhythmic
deposition of enamel.
These are seen as brownish lines that run obliquely across the enamel from
DEJ to the outer surface of enamel.
The striae are wider apart in the middle portion of the enamel, while they are
closer together and more numerous in the cervical enamel due to the slower
deposition of enamel.
In cross-section, these appear as concentric rings.
The lines are less prominent in the enamel formed before birth and more
pronounced in the enamel formed after birth.
Dead Tracts
Injury resulting in death and subsequent loss of odontoblastic process leads to
empty dentinal tubules which become filled with air. Such tubules form dead
tracts.
In ground sections, these tubules appear dark in transmitted light and white in
reflected light.
Because of the convergence of the dentinal tubules towards the pulp, dead
tracts appear narrow at the pulpal end and broader at the cuspal or incisal ends.
Interglobular Dentin
Interglobular dentin is a zone of hypocalcification or hypomineralization seen
in circumpulpal dentin in the crown and occasionally below the Tomes’
granular layer in root dentin.
In ground sections, this appears as dark, star-shaped structure under
transmitted light.
This occurs due to the failure of globules of dentin to fuse into a homogenous
mass during calcification.
The dentinal tubules pass uninterrupted through interglobular dentin.
Peritubular dentin is absent in those portions of the tubules passing through
interglobular dentin.
Secondary Dentin
Secondary dentin is formed after root completion.
It is found bordering the pulp.
It contains lesser number of tubules when compared with that of primary
dentin.
Tubules are more irregular when compared with that of primary dentin.
Continuous deposition of secondary dentin on the floor of the pulp leads to
smaller pulp chambers and narrower root canals.